Abstract:
Four furanoid glycosylated diterpenes (mascaroside I, mascaroside III, mascaroside IV and mascaroside V) were extracted from green grains of Coffea arabica after clean up steps with organic solvents (chloroform/acetone and 80 % ethanol) and concentrated with the aid of macroporous polymeric resins (XAD-2) in column. The identification of compounds has been extensively tested with the aid of hyphenated spectroscopic techniques, such as TLC-MS and ESI-HRMS. The methodology employed was shown to be efficient (81 % recovery after column) and robust (molecular ions of compounds with error <5 ppm), fitting the QuEChERS concept.
