Extraction and enrichment of furanoid glycosylated diterpenes present in green coffee beans: New methodology in the QuEChERS concept

Abstract

Four furanoid glycosylated diterpenes (mascaroside I, mascaroside III, mascaroside IV and mascaroside V) were extracted from green grains of Coffea arabica after clean up steps with organic solvents (chloroform/acetone and 80 % ethanol) and concentrated with the aid of macroporous polymeric resins (XAD-2) in column. The identification of compounds has been extensively tested with the aid of hyphenated spectroscopic techniques, such as TLC-MS and ESI-HRMS. The methodology employed was shown to be efficient (81 % recovery after column) and robust (molecular ions of compounds with error <5 ppm), fitting the QuEChERS concept.